Part:BBa_K4947009
RBS for GmCHS8, expressed in E. coli
This is the ribosome binding sequence for G. max Chalcone Synthase 7.
Description
This is the ribosome binding sequence for G. max Chalcone Synthase 7. This was designed using the CAD-SGE algorithm designed by Jaymin Patel in Farren Isaacs’ lab at Yale University. The algorithm was run repeatedly until the translation initiation rate reached over fifty thousand.
Usage
This part was used in the assembly of the biosynthetic pathway of daidzein. In the construct, it preceded the CDS for G. max Chalcone Synthase 7. This part, along with the rest of the biosynthetic pathway of daidzein, was successfully constructed, cloned into, and integrated into the genome of E. coli DH10Beta.
Experience
This part had little to no difficulty in amplification. It also was an effective ribosome binding site.
References
1. Cross-kingdom expression of synthetic genetic elements promotes discovery of metabolites in the human microbiome. Patel JR, Oh J, Wang S, Crawford JM, Isaacs FJ. Cell. 2022 Apr 28;185(9):1487-1505.e14. doi: 10.1016/j.cell.2022.03.008. Epub 2022 Apr 1. 10.1016/j.cell.2022.03.008 PubMed 35366417 Algorithm: https://cad-sge.com/
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
rbs |